Abstract: Polyetheretherketone (PEEK), as a material for medical implants, has good biocompatibility, but its mechanical properties are relatively low, which limits its wide application in the field of medical bone implants. The addition of carbon fiber (CF) can effectively enhance the mechanical properties of the material. To meet the individualized requirements of bone implantable strength for different patients, in this study, a self-made dual-head fused deposition modeling (FDM) 3D printer was used to prepare PEEK and carbon fiber reinforced polyetheretherketone (CF/PEEK) laminated structure specimens. Through single-factor tests, the printing temperatures of PEEK material and CF/PEEK material were determined to be 410℃ and 420℃ respectively. Based on the response surface method (RSM), taking the layer thickness, the volume ratio (proportion) of PEEK and CF/PEEK, and the printing trajectory Angle (Angle) of CF/PEEK as variables, a three-factor three-level Box-Behnken test was conducted to study its influence on the tensile, bending and interlaminar shear strength of the laminated structure. The results show that the main factors affecting tensile strength and flexural strength are Angle and layer thickness, while the main factor affecting interlaminar shear strength is proportion. The fracture morphology was observed by SEM, and the influencing factors were further analyzed at the microscopic level. This provides a process reference for the regulation of mechanical properties of laminated structures and promots their application in fields such as biomedicine.