Abstract: In recent years, the plastics pollution is becoming more and more serious due to the overuse of fossil-derived transparent films. It is significant to prepare the composite transparent film with excellent comprehensive properties based on green environment-friendly materials. Cellulose is an ideal raw material for the preparation of flexible composite transparent materials because of its advantages of environmental friendliness, renewability and sustainability. In this study, bacterial cellulose (BC) was immersed in phenolic resin (PF) solution, and BC/PF composite transparent film was prepared by hot pressing technology. The effects of phenolic resin concentration and hot-pressing temperature on the microstructure, optical properties, thermal stability, mechanical properties and wettability of BC/PF composite transparent film were investigated. The results show that the BC/PF composite film has a more compact structure and smooth surface when compared with BC film, and its transmittance as high as 88%. The mechanical strength, thermal stability, and waterproof performance of the BC/PF composite film are also significantly improved. The dry strength and wet strength of BC/PF composite film are 2.2 times and 3.4 times higher than that of BC film. This study has scientific guiding significance for alleviating pollution of plastic transparent film and exploring the rapid preparation of green transparent film.