Abstract: In order to further improve the bone inductive properties of bone repair materials, BaTiO₃piezoelectric coating was introduced.First, three kinds of biological coatings of hydroxyapatite (HA), Ti-HA and Ti-HA-BaTiO₃ were prepared on Ti substrates by plasma spraying method. Then, the spray process parameters were optimized by orthogonal test design, the morphology and composition of coatings were characterized by SEM/EDS, and the bonding strength between coating and substrate was tested by scratch test. Finally, the cell culture test was conducted, the cytotoxicity was evaluated by CCK-8 method, and the adhesion morphology of cells was observed under SEM. The results show that cells have pseudopodia, present fusiform and irregular polygons, and adhere to the surface of Ti-HA-BaTiO₃ composite coating; cells spread in good shape, the cytotoxicity level of coating is no higher than Level 1.It follows that Ti-HA-BaTiO₃bio-composites can be used as clinical implant materials.